Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13951] to MBIP - BSA and Azide free
- Suitable for: IHC-P, ICC, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-MBIP antibody [EPR13951] - BSA and Azide free
See all MBIP primary antibodies -
Description
Rabbit monoclonal [EPR13951] to MBIP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250901 is the carrier-free version of ab186750. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250901 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR13951 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDaThis data was developed using ab186750, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution
Lane 1 : Human fetal lung tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 39 kDaThis data was developed using ab186750, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab186750, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MBIP with ab186750 at 1/250 dilution. Secondary antibody: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab186750, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling MBIP with ab186750 at 1/250 dilution. Secondary ab: ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab186750, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling MBIP with ab186750 at 1/500 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488). Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue).
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This data was developed using ab186750, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cell labeling MBIP using ab186750 at 1/400 dilution. Secondary antibody: Goat anti rabbit IgG (FITC) at 1/400 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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