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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway

Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)

Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13951] to MBIP - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-MBIP antibody [EPR13951] - BSA and Azide free
    See all MBIP primary antibodies
  • Description

    Rabbit monoclonal [EPR13951] to MBIP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250901 is the carrier-free version of ab186750. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250901 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR13951
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Images

  • Western blot - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    Western blot - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa



    This data was developed using ab186750, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    Western blot - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution

    Lane 1 : Human fetal lung tissue lysate
    Lane 2 : Human fetal heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 39 kDa



    This data was developed using ab186750, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    This data was developed using ab186750, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MBIP with ab186750 at 1/250 dilution. Secondary antibody: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    This data was developed using ab186750, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling MBIP with ab186750 at 1/250 dilution. Secondary ab: ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    Immunocytochemistry - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    This data was developed using ab186750, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling MBIP with ab186750 at 1/500 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488). Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue).
  • Flow Cytometry - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    Flow Cytometry - Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    This data was developed using ab186750, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cell labeling MBIP using ab186750 at 1/400 dilution. Secondary antibody: Goat anti rabbit IgG (FITC) at 1/400 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
  • Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)
    Anti-MBIP antibody [EPR13951] - BSA and Azide free (ab250901)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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