Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)
![Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)](https://www.abcam.com/ps/products/249/ab249351/Images/ab249351-2-anti-alpha-1-antitrypsin-antibody-epr9090-bsa-and-azide-free-immunocytochemistry.jpg "Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9090] to alpha 1 Antitrypsin - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free
See all alpha 1 Antitrypsin primary antibodies -
Description
Rabbit monoclonal [EPR9090] to alpha 1 Antitrypsin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human alpha 1 Antitrypsin. The exact sequence is proprietary.
Database link: P01009 -
General notes
Ab249351 is the carrier-free version of ab166610. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249351 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9090 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry - Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)This data was developed using ab166610, the same antibody clone in a different buffer formulation.ab166610 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166610 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
-
![Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)](https://www.abcam.com/ps/products/249/ab249351/Images/ab249351-398136-anti-alpha-1-antitrypsin-antibody-epr9090-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)All lanes : Anti-alpha 1 Antitrypsin antibody [EPR9090] (ab166610) at 1/1000 dilution
Lane 1 : Human serum lysate
Lane 2 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?This data was developed using ab166610, the same antibody clone in a different buffer formulation.
-
![Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)](https://www.abcam.com/ps/products/249/ab249351/Images/ab249351-3-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Antitrypsin antibody [EPR9090] - BSA and Azide free (ab249351)
