Anti-GFAP antibody (ab53554)
Key features and details
- Goat polyclonal to GFAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GFAP antibody
See all GFAP primary antibodies -
Description
Goat polyclonal to GFAP -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human GFAP aa 400 to the C-terminus (C terminal) (Cysteine residue).
Database link: P14136-1 -
Positive control
- WB: Human cerebellum and cerebral cortex lysates. Mouse and rat brain lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GFAP antibody (ab53554)All lanes : Anti-GFAP antibody (ab53554) at 0.001 µg/ml
Lane 1 : Human cerebellum tissue lysate (in RIPA buffer)
Lane 2 : Human cerebral cortex tissue lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Western blot - Anti-GFAP antibody (ab53554)Lane 1 : Anti-GFAP antibody (ab53554) at 0.003 µg/ml
Lane 2 : Anti-GFAP antibody (ab53554) at 0.01 µg/ml
Lane 1 : Mouse brain tissue lysate (in RIPA buffer)
Lane 2 : Rat brain tissue lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Western blot - Anti-GFAP antibody (ab53554) This image was courtesy of an annonymous AbreviewAll lanes : Anti-GFAP antibody (ab53554) at 1/1000 dilution
All lanes : Human Tissue lysate - other (Frontal Cortex)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Donkey anti-goat IRDye 800CW conjugated Li-Cor IRdye
Predicted band size: 50 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?
