Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [Nfl21] to 68kDa Neurofilament/NF-L - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free
    See all 68kDa Neurofilament/NF-L primary antibodies

  • Description

    Mouse monoclonal [Nfl21] to 68kDa Neurofilament/NF-L - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SK-N-BE(2) whole cell lysate. Human cerebrospinal fluid. IHC-P: Human, mouse and rat cerebrum tissue.

  • General notes

    ab273479 is the carrier-free version of ab273441.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)
    Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)
    All lanes : Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441) at 1/1000 dilution

    Lane 1 : SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate at 20 µg
    Lane 2 : Human cerebrospinal fluid at 40 µl

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 62 kDa



    This data was developed using ab273441 the same antibody clone in a different buffer formulation.

    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 37 seconds; Lane 2: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)

    This data was developed using ab273441 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)
    Western blot - Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)
    All lanes : Anti-68kDa Neurofilament/NF-L antibody [Nfl21] (ab273441) at 1/1000 dilution

    Lane 1 : Mouse hippocampus tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat hippocampus tissue lysate
    Lane 5 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 62 kDa



    This data was developed using ab273441 the same antibody clone in a different buffer formulation.

    Negative control: liver (PMID: 2120242).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-2: 10 seconds; Lane 3-5: 37 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)

    This data was developed using ab273441 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody - BSA and Azide free (ab273479)

    This data was developed using ab273441 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofilament light with ab273441 at 1/500 (2.116 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab273441 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)
    Anti-68kDa Neurofilament/NF-L antibody [Nfl21] - BSA and Azide free (ab273479)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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