Immunofluorescence of rat cerebellum labeling GFAP with ab254082 (green) at a 1/5000 dilution. Co-labeled with anti-MeCP2 (1/500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.

Immunolabeling of mouse cortical cultures labeling GFAP with ab254082 (green) at a 1/1000 dilution and anti-MAP2 (red).

Immunofluorescence of rat cerebellum tissue labeling GFAP with ab254082 (green) at a 1/1000 dilution. Co-labeled with anti- calbindin (red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.