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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments

Anti-GFAP antibody [ZRF-1] (ab154474)

Price and availability

268 032 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-GFAP antibody [ZRF-1] (ab154474)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [ZRF-1] to GFAP
  • Suitable for: WB, IP
  • Reacts with: Human, Zebrafish
  • Isotype: IgG1

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Overview

  • Product name

    Anti-GFAP antibody [ZRF-1]
    See all GFAP primary antibodies
  • Description

    Mouse monoclonal [ZRF-1] to GFAP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human, Zebrafish
    Does not react with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Human brain and Zebrafish brain homogenates.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    Near homogeneity as judged by SDS-PAGE. ab154474 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    ZRF-1
  • Isotype

    IgG1
  • Light chain type

    lambda
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class II
    • GFAP
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte

Images

  • Western blot - Anti-GFAP antibody [ZRF-1] (ab154474)
    Western blot - Anti-GFAP antibody [ZRF-1] (ab154474)
    All lanes : Anti-GFAP antibody [ZRF-1] (ab154474) at 0.5 µg/ml

    Lane 1 : Human Brain Homogenate lysate
    Lane 2 : Zebrafish Brain Homogenate lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

    Predicted band size: 51 kDa

  • Immunoprecipitation - Anti-GFAP antibody [ZRF-1] (ab154474)
    Immunoprecipitation - Anti-GFAP antibody [ZRF-1] (ab154474)
    Immunoprecipitation Western Blot for ab154474.
    All lanes: ab154474 at 0.5 µg/ml.
    Lane 1: Zebrafish Brain Homogenate lysate 10 µg
    Lane 2: GFAP immunoprecipitated from 1 mg Zebrafish Brain Homogenate lysate 25µl.
    Secondary: Goat polyclonal to Mouse IgG - AP at 1/3000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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