Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP672Y] to GFAP - BSA and Azide free
  • Suitable for: WB, IHC-P, Mass Cytometry, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GFAP antibody [EP672Y] - BSA and Azide free
    See all GFAP primary antibodies

  • Description

    Rabbit monoclonal [EP672Y] to GFAP - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Mouse species is recommended based on IHC and ICC results, we do not guarantee WB for mouse.

  • Tested applications

    Suitable for: WB, IHC-P, Mass Cytometry, ICC/IF, Flow Cyt (Intra)more details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab220820 is the carrier-free version of ab33922.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Mass Cytometry - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
    Mass Cytometry - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820) This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

    Imaging Mass Cytometry™ (IMC™) image of human glioblastoma brain cancer tissue stained with Anti-GFAP antibody [EP672Y]. ab220820 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

    Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)

    This data was developed using ab33922, the same antibody clone in a different buffer formulation.

    ab33922 showing positive staining in Normal human brain tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
    Immunocytochemistry - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)

    This data was developed using ab33922, the same antibody clone in a different buffer formulation.

    Immunocytochemistry analysis of Embryonic mouse primary neural cells labeling GFAP with purified ab33922 at 1:100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. Cells were counterstained with Tau Mouse mAb 1:100 (5 μg/ml). ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) was used as the secondary antibody for the counter stain at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. 

    Confocal image showing positive staining in glial cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820) This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury.

    This data was developed using ab33922, the same antibody clone in a different buffer formulation.

    ICC image of ab33922 stained Rat primary mixed astrocytes culture. The cells were 100% Paraformaldehyde fixed and then incubated in 10% Serum / 0.1M PBS with 10% Donkey serum for 4h. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)

    This data was developed using ab33922, the same antibody clone in a different buffer formulation.

    ab33922 showing positive staining in human Astrocytoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)

    This data was developed using ab33922, the same antibody clone in a different buffer formulation.

    ab33922 showing negative staining in human Meningioma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
    OI-RD Scanning - Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)

    This data was developed using ab33922, the same antibody clone in a different buffer formulation.

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

  • Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)
    Anti-GFAP antibody [EP672Y] - BSA and Azide free (ab220820)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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