Anti-Peripherin antibody (ab4666)
Key features and details
- Rabbit polyclonal to Peripherin
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Peripherin antibody
See all Peripherin primary antibodies -
Description
Rabbit polyclonal to Peripherin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB RatHuman
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Immunogen
Recombinant full length rat peripherin purified from E. coli.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)
Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.
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Western blot - Anti-Peripherin antibody (ab4666)Anti-Peripherin antibody (ab4666) at 1/1000 dilution + Rat spinal cord homogenate
Observed band size: 57 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Peripherin antibody (ab4666)Anti-Peripherin antibody (ab4666) at 1 µg/ml +Recombinant Human Peripherin protein (ab114335) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 secondsSecondary antibody - goat anti-rabbit HRP preasorbed (ab97080)
