Anti-ERC2 antibody (ab27250)
Key features and details
- Rabbit polyclonal to ERC2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-ERC2 antibody
See all ERC2 primary antibodies -
Description
Rabbit polyclonal to ERC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide corresponding to Rat ERC2 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available asab27861) -
Positive control
- This antibody gave a positive signal in Rat brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB RatAll applications MouseHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa.Target
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Function
Thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act together with BSN. May recruit liprin-alpha proteins to the CAZ. -
Cellular localization
Cytoplasm. Cell junction > synapse. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. In neurons, localized to synapses, and colocalizes with PCLO. Localized to the active zone of presynaptic density. - Information by UniProt
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Database links
- Entrez Gene: 26059 Human
- Entrez Gene: 238988 Mouse
- Entrez Gene: 259269 Rat
- SwissProt: O15083 Human
- SwissProt: Q6PH08 Mouse
- SwissProt: Q8K3M6 Rat
- Unigene: 476389 Human
- Unigene: 318004 Mouse
see all -
Alternative names
- Cast antibody
- CAST1 antibody
- CAZ-associated structural protein 1 antibody
see all
Images
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Western blot - Anti-ERC2 antibody (ab27250)Anti-ERC2 antibody (ab27250) at 1 µg/ml + Rat Brain Whole Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 111 kDa
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
Protocols
Datasheets and documents
References (0)
ab27250 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ERC2 antibody (ab27250)Anti-ERC2 antibody (ab27250) at 1 µg/ml + Rat Brain Whole Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 111 kDa
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
