Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23445-28] to Peripherin - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB, IHC-P, IHC-Fr, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free
    See all Peripherin primary antibodies

  • Description

    Rabbit monoclonal [EPR23445-28] to Peripherin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Rat
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human spinal cord, Human lower limb nerve, Human nerve, SH-SY5Y, Mouse dorsal ganglion, Rat dorsal ganglion, Mouse spinal cord, Rat spinal cord, Mouse sciatic nerve, Neuro-2a and PC-12 lysates. IHC-P: Human colon, Mouse colon and Rat colon tissues. IHC-Fr: Mouse and rat colon tissue. ICC: SH-SY5Y and Neuro-2a cells. Flow:: SH-SY5Y and Neuro-2a cells. IP: Human spinal cord lysate.

  • General notes

    ab269861 is the carrier-free version of ab246502. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Peripherin with ab246502 at 1/10,000 dilution (0.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in rat colon is observed. The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Peripherin with ab246502 at 1/10,000 dilution (0.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in mouse colon is observed. The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Peripherin with ab246502 at 1/2000 dilution (0.227 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in human colon (PMID: 26469323). The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Immunoprecipitation - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Immunoprecipitation - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Peripherin was immunoprecipitated from 0.35 mg Human spinal cord lysate with ab246502 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246502 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human spinal cord lysate 10ug

    Lane 2: ab246502 IP in Human spinal cord lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246502 in Human spinal cord lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 40 seconds.

    The 58KD isoform of peripherin observed is consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Flow Cytometry - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Flow Cytometry - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling Peripherin with ab246502 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Flow Cytometry - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Flow Cytometry - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Peripherin with ab246502 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Immunohistochemistry (Frozen sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Immunohistochemistry (Frozen sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat colon tissue labeling Peripherin with ab246502 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on the ganglion cells in rat colon is observed. The nuclear counter stain is DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Immunohistochemistry (Frozen sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Immunohistochemistry (Frozen sections) - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse colon tissue labeling Peripherin with ab246502 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on the ganglion cells in mouse colon is observed. The nuclear counter stain is DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Peripherin with ab246502 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. Tubulin is detected using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The nuclear counter stain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

    Immunofluorescent analysis of4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling Peripherin with ab246502 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line. Tubulin is detected using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The nuclear counter stain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).

  • Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
    Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)

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