Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Peripherin antibody [EPR23445-28] (ab246502)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23445-28] to Peripherin
Suitable for: WB, Flow Cyt, IP, IHC-P, IHC-Fr, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Peripherin antibody [EPR23445-28]
See all Peripherin primary antibodies
Description

Rabbit monoclonal [EPR23445-28] to Peripherin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
ICC/IF	Mouse Human
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human spinal cord, Human lower limb nerve, Human nerve, SH-SY5Y, Mouse dorsal ganglion, Rat dorsal ganglion, Mouse spinal cord, Rat spinal cord, Mouse sciatic nerve, Neuro-2a and PC-12 lysates. IHC-P: Human colon, Mouse colon and Rat colon tissues. IHC-Fr: Mouse and rat colon tissue. ICC: SH-SY5Y and Neuro-2a cells. Flow:: SH-SY5Y and Neuro-2a cells. IP: Human spinal cord lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23445-28
Isotype

IgG
Research areas

Western blot - Anti-Peripherin antibody [EPR23445-28] (ab246502)

All lanes : Anti-Peripherin antibody [EPR23445-28] (ab246502) at 1/1000 dilution

Lane 1 : Mouse dorsal ganglion lysate
Lane 2 : Rat dorsal ganglion lysate
Lane 3 : Mouse spinal cord lysate
Lane 4 : Rat spinal cord lysate
Lane 5 : Mouse sciatic nerve lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 28,45,56,58 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 26 seconds.

The 58KD, 56KD, 45KD and 28KD isoforms of peripherin observed are consistent with what has been described in the literature (PMID: 20587592,18287500,17475883).
Western blot - Anti-Peripherin antibody [EPR23445-28] (ab246502)

All lanes : Anti-Peripherin antibody [EPR23445-28] (ab246502) at 1/1000 dilution

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 45,56,58 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 6 seconds.

The 58KD, 56KD and 45KD isoforms of peripherin observed are consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).
Western blot - Anti-Peripherin antibody [EPR23445-28] (ab246502)

All lanes : Anti-Peripherin antibody [EPR23445-28] (ab246502) at 1/1000 dilution

Lane 1 : Human spinal cord lysate
Lane 2 : Human lower limb nerve lysate
Lane 3 : Human nerve lysate
Lane 4 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 5 : Human kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) or VeriBlot for IP secondary antibody (HRP) (ab131366) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 45,56,58 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 37 seconds.

The 58KD, 56KD, and 45KD isoforms of peripherin observed are consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).

Negative control: Kidney (PMID: 15132161).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Peripherin with ab246502 at 1/10,000 dilution (0.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in rat colon. The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Peripherin with ab246502 at 1/10,000 dilution (0.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in mouse colon. The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Peripherin with ab246502 at 1/2000 dilution (0.227 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in human colon (PMID: 26469323). The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Peripherin was immunoprecipitated from 0.35 mg Human spinal cord lysate with ab246502 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246502 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Human spinal cord lysate 10ug

Lane 2: ab246502 IP in Human spinal cord lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246502 in Human spinal cord lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 40 seconds.

The 58KD isoform of peripherin observed is consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).
Flow Cytometry - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling Peripherin with ab246502 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Peripherin with ab246502 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat colon tissue labeling Peripherin with ab246502 at 1/250 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on the ganglion cells in rat colon is observed. The nuclear counter stain is DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse colon tissue labeling Peripherin with ab246502 at 1/250 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on the ganglion cells in mouse colon is observed. The nuclear counter stain is DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunofluorescent analysis of4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Peripherin with ab246502 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. Tubulin is detected using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (Red). The nuclear counter stain is DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling Peripherin with ab246502 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line. Tubulin is detected using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (Red). The nuclear counter stain is DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 dilution.
Anti-Peripherin antibody [EPR23445-28] (ab246502)

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