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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments

Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)

Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [NF421 + NFL/736] to Neurofilament
  • Suitable for: Flow Cyt, ICC, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Neurofilament antibody [NF421 + NFL/736]
    See all Neurofilament primary antibodies
  • Description

    Mouse monoclonal [NF421 + NFL/736] to Neurofilament
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human Neurofilament aa 1 to the C-terminus.
    Database link: P12036

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human and rat cerebellum tissues. HEK293 cells.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab215903 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    NF421 + NFL/736
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat cerebellum tissue labeling Neurofilament with ab215903 at 1 μg/ml dilution.

  • Immunocytochemistry - Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)
    Immunocytochemistry - Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)
    Immunofluorescence staining of HEK293 cells usingab215903 followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).
     
     
     
     
     
     
     
     

     

  • Flow Cytometry - Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)
    Flow Cytometry - Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)
    Flow Cytometric Analysis of HEK293 cells using ab215903 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
     
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebellum tissue labeling Neurofilament with ab215903 at 1 μg/ml dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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