Anti-NPTX2 antibody (ab69858)
Key features and details
- Rabbit polyclonal to NPTX2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NPTX2 antibody
See all NPTX2 primary antibodies -
Description
Rabbit polyclonal to NPTX2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A 16 amino acid peptide near the center of the human NPTX2
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTX2 antibody (ab69858)
Immunohistochemical analysis of paraffin-embedded human brain using ab69858 at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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All lanes : Anti-NPTX2 antibody (ab69858) at 1 µg/ml
Lane 1 : Mouse Brain tissue lysate
Lane 2 : Mouse Brain tissue lysate with Blocking peptide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDaDiluting buffer: 5% NFDM/TBST.
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Lane 1 : Anti-NPTX2 antibody (ab69858) at 0.5 µg
Lane 2 : Anti-NPTX2 antibody (ab69858) at 1 µg/ml
All lanes : Mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 47 kDaDiluting buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde fixed human brain tissue labeling NPTX2 with ab69858 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).