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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments

Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)

Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [NF421 + NFL/736] to Neurofilament - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free
    See all Neurofilament primary antibodies
  • Description

    Mouse monoclonal [NF421 + NFL/736] to Neurofilament - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Pig
  • Immunogen

    Recombinant full length protein corresponding to Human Neurofilament aa 1 to the C-terminus.
    Database link: P12036

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human and rat cerebellum tissues; ICC and Flow Cyt: HEK-293 cells.
  • General notes

    ab213128 is a carrier-free version of ab215903.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab21593 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    NF421 + NFL/736
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Disease
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development

Images

  • Immunocytochemistry - Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)
    Immunocytochemistry - Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)
    This image was produced using ab215903, the same clone but in a different formulation. HEK-293 (human epithelial cell line from embryonic kidney) stained for Naurofilament using ab215903 at 2 ug/ml.
  • Flow Cytometry - Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)
    Flow Cytometry - Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)
    This image was produced using ab215903, the same clone but in a different formulation. Flow Cytometric analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells staining neurofilament using ab215903 at 2ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebellum tissue labeling Neurofilament with ab215903 at 1 μg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215903).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] - BSA and Azide free (ab213128)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat cerebellum tissue labeling Neurofilament with ab215903 at 1 μg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215903).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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