Anti-GFAP antibody - Affinity Purified (ab211271)
Key features and details
- Rabbit polyclonal to GFAP - Affinity Purified
- Suitable for: WB, IHC-FrFl
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GFAP antibody - Affinity Purified
See all GFAP primary antibodies -
Description
Rabbit polyclonal to GFAP - Affinity Purified -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-FrFl RatWB MouseRat
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Immunogen
Recombinant full length protein corresponding to GFAP.
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General notes
In some cases, the antibody may appear red in color. This is due to small amounts of hemolysis, and does not affect antibody performance.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
The antibody was made against full length recombinant GFAP based on the human sequence expressed in and purified from E. coli. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry - Free Floating - Anti-GFAP antibody - Affinity Purified (ab211271)
Immunohistochemistry (Free Floating) analysis of rat cerebellum staining GFAP with ab211271 (1/5000) in green and MeCP2 with a mouse monoclonal antibody (1/500) in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45μM, and free-floating sections were stained with above antibodies.
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Western blot - Anti-GFAP antibody - Affinity Purified (ab211271)Lane 1 : Protein ladder
Lanes 2-5 : Anti-GFAP antibody - Affinity Purified (ab211271) at 1/5000 dilution
Lane 2 : Rat brain
Lane 3 : Rat spinal cord
Lane 4 : Mouse brain
Lane 5 : Mouse spinal cordStrong band at about 50kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
