Anti-Nestin antibody (ab134017)
Key features and details
- Chicken polyclonal to Nestin
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse
- Isotype: IgY
Overview
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Product name
Anti-Nestin antibody
See all Nestin primary antibodies -
Description
Chicken polyclonal to Nestin -
Host species
Chicken -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Three synthetic peptides, conjugated to KLH, corresponding to regions within Mouse Nestin (NP_057910).
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Positive control
- e13 mouse brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
IgY fractions were purified from the yolks and IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 300 µg/ml, and the preparation filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Immunocytochemistry - Anti-Nestin antibody (ab134017)
Immunocytochemistry/Immunofluorescence analysis of 3T3 cells labelling Nestin (green) with ab134017. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin I, Blue - Hoechst stain for nuclei.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)Immunohistochemical analysis of a tissue section through an e13 mouse brain labelling Nestin (green staining) in neural stem cells of the ventricular zone of the CNS with ab134017 at 1/10000 dilution. DAPI (blue staining) allows visualization of nuclei.
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Western blot - Anti-Nestin antibody (ab134017)Anti-Nestin antibody (ab134017) at 1/10000 dilution + neonatal mouse brain
Predicted band size: 177 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody (ab134017)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Nestin (red) with ab134017. Tissue was fixed with 10% formalin. Green - rabbit anti-Synaptotagmin, Blue - Hoechst stain for nuclei.
