Anti-C12orf34 antibody (ab122626)
Key features and details
- Rabbit polyclonal to C12orf34
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C12orf34 antibody -
Description
Rabbit polyclonal to C12orf34 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PSIHSLLYQL NQQCQAPGAA PPACQGMAIP HPSPAKHGPV PSFPSMAYSA AAGLPDCRKG TELGQGATQA LTLAGAAKPA GYADSGLDYL LWPQKP, corresponding to amino acids 197-292 of Human C12orf34.
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Positive control
- IHC-P: Human stomach, testis, and cerebral cortex tissues; ICC: U 251 MG cells; WB: SH-SY5Y whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-C12orf34 antibody (ab122626)Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane, mitochondria and focal adhesions. Recommended concentration of ab122626 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-C12orf34 antibody (ab122626)Anti-C12orf34 antibody (ab122626) + SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of stomach tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of cerebral cortex tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of testis tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of skeletal muscle tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol. No positivity in myocytes as expected.