Alexa Fluor® 568 Anti-Oct4 antibody [EPR17929] (ab209598)
![Alexa Fluor® 568 Anti-Oct4 antibody [EPR17929] (ab209598)](https://www.abcam.com/ps/products/209/ab209598/Images/ab209598-260424-anti-oct4-antibody-epr17929-alexa-fluor-568-immunofluorescence.jpg "Alexa Fluor® 568 Anti-Oct4 antibody [EPR17929] (ab209598)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 568 Rabbit monoclonal [EPR17929] to Oct4
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Overview
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Product name
Alexa Fluor® 568 Anti-Oct4 antibody [EPR17929]
See all Oct4 primary antibodies -
Description
Alexa Fluor® 568 Rabbit monoclonal [EPR17929] to Oct4 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 568. Ex: 578nm, Em: 603nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: mouse Embryonic Stem cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17929 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Oct4 antibody [EPR17929] (ab209598)
ab209598 staining Oct4 in mouse Embryonic Stem cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209598 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in mouse Embryonic Stem cells fixed with 4% formaldehyde (10 min).
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![Alexa Fluor® 568 Anti-Oct4 antibody [EPR17929] (ab209598)](https://www.abcam.com/ps/products/209/ab209598/Images/ab209598-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 568 Anti-Oct4 antibody [EPR17929] (ab209598)
