Anti-Oct4 antibody [GT486] (ab184665)
![Anti-Oct4 antibody [GT486] (ab184665)](https://www.abcam.com/ps/products/184/ab184665/Images/ab184665-215578-anti-oct4-antibody-gt486-immunofluorescence.jpg "Anti-Oct4 antibody [GT486] (ab184665)")
Key features and details
- Mouse monoclonal [GT486] to Oct4
- Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
-
Product name
Anti-Oct4 antibody [GT486]
See all Oct4 primary antibodies -
Description
Mouse monoclonal [GT486] to Oct4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB MouseHuman
-
Immunogen
Recombinant fragment within Human Oct4 aa 1-182. The exact sequence is proprietary.
Database link: Q01860 -
Positive control
- Oct4 transfected 293T and HeLa cells; NT2D1 whole cell lysates; mouse and Human ESC whole cell lysates; Human embryonic stem cells and BT483 xenograft tissue.
-
General notes
This product was changed from ascites to tissue culture supernatant on 17th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
GT486 -
Isotype
IgG2b -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [GT486] (ab184665)
Immunofluorescence analysis of 4% paraformaldehyde-fixed Human embryonic stem cells labeling Oct4 with ab184665 at 1/200 dilution (green). Hoechst 33342 staining is shown in blue.
-
![Immunoprecipitation - Anti-Oct4 antibody [GT486] (ab184665)](https://www.abcam.com/ps/products/184/ab184665/Images/ab184665-215587-anti-oct4-antibody-gt486-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Oct4 antibody [GT486] (ab184665)Immunoprecipitation of Oct4 transfected 293T cell lysate followed by detection of Oct4 with ab184665 at 1/1000 dilution.
Lane 1; Cell lysate/extract of transfected 293T cells.Lane 2; Cell lysate/extract of transfected 293T cells precipitated with 2 μg of preimmune mouse IgG (control)
Lane 3; Cell lysate/extract of transfected 293T cells precipitated using 2 µg of ab184665.
Detection utilised anti-mouse IgG. Samples analysed by 12% SDS-PAGE. -
![Western blot - Anti-Oct4 antibody [GT486] (ab184665)](https://www.abcam.com/ps/products/184/ab184665/Images/ab184665-215585-anti-oct4-antibody-gt486-western-blot.jpg)
Western blot - Anti-Oct4 antibody [GT486] (ab184665)Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution + Mouse ESC whole cell lysate at 20 µg
Predicted band size: 39 kDa10% SDS PAGE
-
![Western blot - Anti-Oct4 antibody [GT486] (ab184665)](https://www.abcam.com/ps/products/184/ab184665/Images/ab184665-215583-anti-oct4-antibody-gt486-western-blot.jpg)
Western blot - Anti-Oct4 antibody [GT486] (ab184665)Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 39 kDa10% SDS PAGE
-
![Western blot - Anti-Oct4 antibody [GT486] (ab184665)](https://www.abcam.com/ps/products/184/ab184665/Images/ab184665-215582-anti-oct4-antibody-gt486-western-blot.jpg)
Western blot - Anti-Oct4 antibody [GT486] (ab184665)All lanes : Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution
Lane 1 : Non-transfected HeLa whole cell lysates
Lane 2 : OCT3/4 transfected HeLa whole cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa10% SDS PAGE
-
![Western blot - Anti-Oct4 antibody [GT486] (ab184665)](https://www.abcam.com/ps/products/184/ab184665/Images/ab184665-215581-anti-oct4-antibody-gt486-western-blot.jpg)
Western blot - Anti-Oct4 antibody [GT486] (ab184665)Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution + Human ESC whole cell lysate/extract at 30 µg
Predicted band size: 39 kDa10 % SDS-PAGE
-
![Flow Cytometry - Anti-Oct4 antibody [GT486] (ab184665)](https://www.abcam.com/ps/products/184/ab184665/Images/ab184665-215580-anti-oct4-antibody-gt486-flow-cytometry.jpg)
Flow Cytometry - Anti-Oct4 antibody [GT486] (ab184665)Flow cytometric analysis of Human embryonic stem cells labeling Oct4 with ab184665 at 1/50 dilution (green). The isotype control was at 1/50 dilution (Black).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (ab184665)](https://www.abcam.com/ps/products/184/ab184665/Images/ab184665-215579-anti-oct4-antibody-gt486-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (ab184665)Immunohistochemical analysis of paraffin-embedded BT483 (human) xenograft tissue labeling Oct4 with ab184665 at 1/200 dilution.
