HRP Anti-Oct4 antibody [EPR2054] (ab194076)
![HRP Anti-Oct4 antibody [EPR2054] (ab194076)](https://www.abcam.com/ps/products/194/ab194076/Images/ab194076-234989-anti-oct4-antibody-epr2054-hrp-immunohistochemistry.jpg "HRP Anti-Oct4 antibody [EPR2054] (ab194076)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR2054] to Oct4
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Oct4 antibody [EPR2054]
See all Oct4 primary antibodies -
Description
HRP Rabbit monoclonal [EPR2054] to Oct4 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human testis (Seminoma) tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 5.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR2054 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Oct4 antibody [EPR2054] (ab194076)
IHC image of Oct4 staining in a section of formalin-fixed paraffin-embedded human testis (Seminoma)*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab194076 at 1µg/ml. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![HRP Anti-Oct4 antibody [EPR2054] (ab194076)](https://www.abcam.com/ps/products/194/ab194076/Images/ab194076-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Oct4 antibody [EPR2054] (ab194076)
