Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2054] to Oct4 - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Oct4 antibody [EPR2054] - BSA and Azide free
    See all Oct4 primary antibodies

  • Description

    Rabbit monoclonal [EPR2054] to Oct4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Seminoma, NCCIT and HeLa cell lysates; Human ovarian dysgerminoma tissue.

  • General notes

    ab222233 is the carrier-free version of ab109183 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab222233 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human seminoma tissue sections labeling Oct4 - with Purified ab109183 at 1:600 dilution (1 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

  • Immunoprecipitation - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunoprecipitation - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 at 1/50 immunoprecipitating Oct4 in NCCIT (human pluripotent embryonal carcinoma) whole cell lysate observed at 39 KDa (lanes 1 and 2).

    Lane 1 (input): NCCIT whole cell lysate, 10μg

    Lane 2 (+): ab109183 + NCCIT whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109183 in NCCIT whole cell lysate

    For western blotting, ab109183 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used for detection antibody at 1/10000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

     

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183, at 1:500 dilution, staining Oct4 in paraffin-embedded Human ovarian dysgerminoma by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 showing negative staining in Colonic adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 showing negative staining in Skeletal muscle tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 showing negative staining in Normal liver tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 showing positive staining in Seminoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 showing positive staining in Fetal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 showing positive staining in Embryonal carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

    Unpurified ab109183 showing negative staining in Hepatocellular carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    OI-RD Scanning - Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109183).

  • Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
    Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)

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