Anti-Oct4 antibody (ab27985)
Key features and details
- Goat polyclonal to Oct4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Oct4 antibody
See all Oct4 primary antibodies -
Description
Goat polyclonal to Oct4 -
Host species
Goat -
Specificity
According to the UniProt database Oct4 is mainly localized in the nucleus but can also occur in the cytoplasm: UniProtKB - Q01860 -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human Oct4 aa 300 to the C-terminus (C terminal).
(Peptide available asab20650)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Affinity Purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Oct4 antibody (ab27985)All lanes : Anti-Oct4 antibody (ab27985) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat IgG secondary antibody (ab28445) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?The antibody recognised a band corresponding to Oct4 in mouse and human embryonic stem cell lysates.
