Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [2D3] to BRD7 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BRD7 antibody [2D3] - BSA and Azide free
    See all BRD7 primary antibodies

  • Description

    Rat monoclonal [2D3] to BRD7 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa, C6, RAW264.7, PC-12, MCF7 and PC-3 whole cell lysate. Mouse brain tissue lysate. IHC-P: Human colon carcinoma tissue. Mouse and rat testis tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255776 is the carrier-free version of ab252820. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BRD7 with ab252820 at 5.67µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat testis tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRD7 with ab252820 at 5.67µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse testis tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling BRD7 with ab252820 at 5.67µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on human colon carcinoma tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

  • Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
    Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
    All lanes : Anti-BRD7 antibody [2D3] (ab252820) at 0.567 µg/ml

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma)
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 74 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

  • Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
    Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
    All lanes : Anti-BRD7 antibody [2D3] (ab252820) at 0.567 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 74 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 15 secs; Lane 3: 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

  • Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
    Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)

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