Astana Biomed Group, an authorized Abcam distributor in Central Asia

Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)

Key features and details

  • Alexa Fluor® 647 Mouse monoclonal [TAL 1B5] to HLA-DR
  • Suitable for: ICC/IF, IHC-Fr, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Isotype: IgG1

Overview

  • Product name

    Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5]
    See all HLA-DR primary antibodies

  • Description

    Alexa Fluor® 647 Mouse monoclonal [TAL 1B5] to HLA-DR

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Raji cells IHC-Fr: normal human tonsil tissue sections Flow Cyt: Raji cells

  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    TAL 1B5

  • Myeloma

    P3-NS1/1-Ag4-1

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)
    Flow Cytometry - Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)

    Overlay histogram showing Raji cells stained with ab223907 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223907, 1/50000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Mouse IgG1 (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)
    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)

    IHC image of HLA DR staining in a section of frozen normal human tonsil*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab223907 at 1/1000 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)

    ab223907 staining HLA DR in Raji cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab223907 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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