Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3692] to HLA-DR - Low endotoxin, Azide free
  • Suitable for: Flow Cyt (Intra), Mass Cytometry, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free
    See all HLA-DR primary antibodies

  • Description

    Rabbit monoclonal [EPR3692] to HLA-DR - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), Mass Cytometry, ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Recombinant fragmentDoes not react with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T cells transfected with a human HLA-DR expression vector containing a his-tag whole cell lysate. Flow Cyt (intra): Raji cells. ICC/IF: Hut-78 cells. IMC: Human tonsil tissue

  • General notes

    ab215985 is the carrier-free version of ab92511.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 1.67 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3692

  • Isotype

    IgG

  • Research areas

Images

  • Mass Cytometry - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Mass Cytometry - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985) This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

    Imaging Mass Cytometry™ (IMC™) image of human tonsil tissue stained with Anti-HLA-DR antibody [EPR3692]. ab215985 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

    Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

  • Flow Cytometry - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Flow Cytometry - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

    Overlay histogram showing Raji cells stained with ab92511 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92511, 1/50) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92511).

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

    ab92511 staining HLA-DR in HuT-78 (human Sezary syndrome) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab92511 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

    ab92511 showing positive staining in Normal liver vessels tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92511).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

    ab92511 showing positive staining in Normal skin vessels tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92511).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

    ab92511 showing positive staining in Endometrial carcinoma vessels tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92511).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    OI-RD Scanning - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

    This IHC data was generated using the same anti-HLA DR antibody clone, EPR3692, in a different buffer formulation (cat# ab92511).

    ab92511 staining HLA-DR in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)
    Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (ab215985)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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