Anti-HLA-DR antibody [DA6.147] (ab238469)
![Anti-HLA-DR antibody [DA6.147] (ab238469)](https://www.abcam.com/ps/products/238/ab238469/Images/ab238469-352429-ab238469AP47332753pcMilkPublish.jpg "Anti-HLA-DR antibody [DA6.147] (ab238469)")
Key features and details
- Mouse monoclonal [DA6.147] to HLA-DR
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-HLA-DR antibody [DA6.147]
See all HLA-DR primary antibodies -
Description
Mouse monoclonal [DA6.147] to HLA-DR -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. Daudi (human Burkitt's lymphoma cell line) cells
-
Positive control
- IHC-P: Human breast and tonsil tissue. WB: Raji and Ramos whole cell lysate.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
DA6.147 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Western blot - Anti-HLA-DR antibody [DA6.147] (ab238469)All lanes : Anti-HLA-DR antibody [DA6.147] (ab238469) at 1 µg/ml
Lane 1 : Raji whole cell lysate
Lane 2 : Ramos whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 29 kDaThis blot was produced using a 4-12% Bis-tris under the MES buffer system. The gel was run at 200V for m5 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab238469 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [DA6.147] (ab238469)](https://www.abcam.com/ps/products/238/ab238469/Images/ab238469-2-anti-hladr-da6147-ihcp-human-breast1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [DA6.147] (ab238469)IHC image of HLA-DR staining in a section of formalin-fixed paraffin-embedded normal human breast* performed on a Leica BOND system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab238469, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [DA6.147] (ab238469)](https://www.abcam.com/ps/products/238/ab238469/Images/ab238469-1-anti-hladr-da6147-ihcp-human-tonsil1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [DA6.147] (ab238469)IHC image of HLA-DR staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BOND system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab238469, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
