Anti-HLA-DR antibody [EPR21208] (ab213305)
![Anti-HLA-DR antibody [EPR21208] (ab213305)](https://www.abcam.com/ps/products/213/ab213305/Images/ab213305-296073-anti-hla-dr-antibody-epr21208-immunohistochemistry.jpg "Anti-HLA-DR antibody [EPR21208] (ab213305)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [EPR21208] to HLA-DR
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-HLA-DR antibody [EPR21208]
See all HLA-DR primary antibodies -
Description
Human monoclonal [EPR21208] to HLA-DR -
Host species
Human -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Recombinant fragment.
Database link: P01903 -
Positive control
- IHC-P: FFPE human spleen tissue sections. ICC: Raji cell line.
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant antibody.
Human monoclonal antibody.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR21208 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR21208] (ab213305)
IHC image of HLA-DR staining in a section of formalin-fixed paraffin-embedded normal human spleen* performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213305, 0.5ug/ml, for 15 mins at room temperature.
An HRP-conjugated goat anti-Human IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR21208] (ab213305)](https://www.abcam.com/ps/products/213/ab213305/Images/ab213305-296074-anti-hla-dr-antibody-epr21208-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR21208] (ab213305)ab213305 staining HLA-DR in Raji cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213305 at 0.5ugml then detected with an Alexa Fluor® 488 goat anti-human secondary antibody at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Anti-HLA-DR antibody [EPR21208] (ab213305)](https://www.abcam.com/ps/products/213/ab213305/Images/ab213305-3-benefits-of-recombinant-antibodies.png)
Anti-HLA-DR antibody [EPR21208] (ab213305)
