Anti-MHC Class II antibody [OX3] (ab243843)
![Anti-MHC Class II antibody [OX3] (ab243843)](https://www.abcam.com/ps/products/243/ab243843/Images/ab243843-2-ab243843MousemonoclonalOX3toMHCclassIILewisratsplenocytesflowcytometry.jpg "Anti-MHC Class II antibody [OX3] (ab243843)")
Key features and details
- Mouse monoclonal [OX3] to MHC Class II
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-MHC Class II antibody [OX3]
See all MHC Class II primary antibodies -
Description
Mouse monoclonal [OX3] to MHC Class II -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Rat splenocytes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX3 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-MHC Class II antibody [OX3] (ab243843)
Flow cytometry staining of Lewis rat splenocytes with ab243843 (right) or mouse IgG1 kappa (ab170190) isotype control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab243843) or mouse IgG1 kappa (ab170190) isotype (1x106 in 100 μl at 0.008 μg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD45R PE.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live lymphocytes.