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Signal Transduction Protein Phosphorylation Ser / Thr Phosphatases

Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)

Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15177] to NXN/NRX - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free
    See all NXN/NRX primary antibodies
  • Description

    Rabbit monoclonal [EPR15177] to NXN/NRX - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251080 is the carrier-free version of ab191429 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251080 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as NXN

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15177
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear

Images

  • Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    All lanes : Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution

    Lane 1 : 293 lysate
    Lane 2 : HepG2 lysate
    Lane 3 : HeLa lysate
    Lane 4 : SH-SY5Y lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab191429, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Flow Cytometry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    This data was developed using ab191429, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of NIH/3T3 (mouse embryo) labelling NXN/NRX with purified ab191429 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
  • Immunocytochemistry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Immunocytochemistry - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling NXN/NRX with ab191429 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution; 2. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Western blot - Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    All lanes : Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab191429, the same antibody clone in a different buffer formulation.

     

  • Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
    Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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