Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free (ab251080)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15177] to NXN/NRX - BSA and Azide free
- Suitable for: Flow Cyt, ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NXN/NRX antibody [EPR15177] - BSA and Azide free
See all NXN/NRX primary antibodies -
Description
Rabbit monoclonal [EPR15177] to NXN/NRX - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251080 is the carrier-free version of ab191429 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251080 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as NXN
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR15177 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution
Lane 1 : 293 lysate
Lane 2 : HepG2 lysate
Lane 3 : HeLa lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab191429, the same antibody clone in a different buffer formulation.
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This data was developed using ab191429, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of NIH/3T3 (mouse embryo) labelling NXN/NRX with purified ab191429 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling NXN/NRX with ab191429 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution; 2. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
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This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab191429, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab191429, the same antibody clone in a different buffer formulation.
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