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Anti-PPM1A antibody [7F12] (ab135249)

Key features and details

  • Mouse monoclonal [7F12] to PPM1A
  • Suitable for: WB, ELISA, Flow Cyt
  • Knockout validated
  • Reacts with: Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-PPM1A antibody [7F12]
    See all PPM1A primary antibodies

  • Description

    Mouse monoclonal [7F12] to PPM1A

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Purified recombinant fragment corresponding to amino acids 202-382 of Human PPM1A, expressed in E. coli.

  • Positive control

    • Human PPM1A recombinant protein. Cell lysate from PPM1A transfected HEK293 cells. Cell lysates from Jurkat, A431, HeLa, HEK293, Raji, MCF-7 and COS7 cells. HeLa cells.

Properties

Images

  • Western blot - Anti-PPM1A antibody [7F12] (ab135249)
    Western blot - Anti-PPM1A antibody [7F12] (ab135249)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PPM1A knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab135249 observed at 42 kDa. Red - loading control, ab18251, observed at 37 kDa.

    ab135249 was shown to specifically react with PPM1A when PPM1A knockout samples were used. Wild-type and PPM1A knockout samples were subjected to SDS-PAGE. ab135249 at a dilution of 1/500 and ab18251 (loading control to alpha Tubulin) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • ELISA - Anti-PPM1A antibody [7F12] (ab135249)
    ELISA - Anti-PPM1A antibody [7F12] (ab135249)
    ELISA analysis using ab135249 with different concentrations of antigen.
  • Western blot - Anti-PPM1A antibody [7F12] (ab135249)
    Western blot - Anti-PPM1A antibody [7F12] (ab135249)
    Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution + Human PPM1A recombinant protein.

    Predicted band size: 42.4 kDa

  • Western blot - Anti-PPM1A antibody [7F12] (ab135249)
    Western blot - Anti-PPM1A antibody [7F12] (ab135249)
    All lanes : Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PPM1A (amino acids 202-382)-hIgGFc transfected HEK293 cell lysate.

    Predicted band size: 42.4 kDa

  • Western blot - Anti-PPM1A antibody [7F12] (ab135249)
    Western blot - Anti-PPM1A antibody [7F12] (ab135249)
    All lanes : Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution

    Lanes 1-2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : Raji cell lysate
    Lane 7 : MCF-7 cell lysate
    Lane 8 : COS-7 (African green monkey kidney fibroblast-like cell line)ncell lysate

    Predicted band size: 42.4 kDa

  • Flow Cytometry - Anti-PPM1A antibody [7F12] (ab135249)
    Flow Cytometry - Anti-PPM1A antibody [7F12] (ab135249)
    Flow cytometric analysis of HeLa cells using ab135249 at 1/200 dilution (green) or a negative control (red).

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