Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [7F12] to PPM1A
Suitable for: WB, ELISA, Flow Cyt
Knockout validated
Reacts with: Human, African green monkey
Isotype: IgG1

Product name

Anti-PPM1A antibody [7F12]
See all PPM1A primary antibodies
Description

Mouse monoclonal [7F12] to PPM1A
Host species

Mouse
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Purified recombinant fragment corresponding to amino acids 202-382 of Human PPM1A, expressed in E. coli.
Positive control
- Human PPM1A recombinant protein. Cell lysate from PPM1A transfected HEK293 cells. Cell lysates from Jurkat, A431, HeLa, HEK293, Raji, MCF-7 and COS7 cells. HeLa cells.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS

Protein stabiliser at 0.5%.
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

7F12
Isotype

IgG1
Research areas

Western blot - Anti-PPM1A antibody [7F12] (ab135249)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PPM1A knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab135249 observed at 42 kDa. Red - loading control, ab18251, observed at 37 kDa.

ab135249 was shown to specifically react with PPM1A when PPM1A knockout samples were used. Wild-type and PPM1A knockout samples were subjected to SDS-PAGE. ab135249 at a dilution of 1/500 and ab18251 (loading control to alpha Tubulin) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
ELISA - Anti-PPM1A antibody [7F12] (ab135249)

ELISA analysis using ab135249 with different concentrations of antigen.
Western blot - Anti-PPM1A antibody [7F12] (ab135249)

Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution + Human PPM1A recombinant protein.

Predicted band size: 42.4 kDa
Western blot - Anti-PPM1A antibody [7F12] (ab135249)

All lanes : Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : PPM1A (amino acids 202-382)-hIgGFc transfected HEK293 cell lysate.

Predicted band size: 42.4 kDa
Western blot - Anti-PPM1A antibody [7F12] (ab135249)

All lanes : Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution

Lanes 1-2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : Raji cell lysate
Lane 7 : MCF-7 cell lysate
Lane 8 : COS-7 (African green monkey kidney fibroblast-like cell line)ncell lysate

Predicted band size: 42.4 kDa
Flow Cytometry - Anti-PPM1A antibody [7F12] (ab135249)

Flow cytometric analysis of HeLa cells using ab135249 at 1/200 dilution (green) or a negative control (red).

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