Anti-NSP1 antibody [32D6]

Mouse monoclonal [32D6] to NSP1

Mouse

This antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for NSP1, strain RS453, which expresses a shortened isoform of NSP1, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype NSP1 is 86.5 kDa, but NSP1 has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype NSP1 was 108 kDa, whereas the short isoform of NSP1 in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein NSP1.

Suitable for: ICC/IF, WBmore details

Reacts with: Saccharomyces cerevisiae

This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

Liquid

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant

Tissue culture supernatant

Sterile filtered.

This antibody is a useful marker of yeast nuclear pores.

Monoclonal

32D6

IgG

• Tags & Cell Markers
• Subcellular Markers
• Nucleus
• Nuclear Envelope

• Tags & Cell Markers
• Subcellular Markers
• Nucleus
• Nuclear Pore