Anti-Nestin antibody [Rat 401] - BSA and Azide free (ab264111)
![Anti-Nestin antibody [Rat 401] - BSA and Azide free (ab264111)](https://www.abcam.com/ps/products/264/ab264111/Images/ab264111-355597-AntiNestin-antibody-Rat-401--BSA-and-Azide-free-immunohistochemistry-brain-rat.jpg "Anti-Nestin antibody [Rat 401] - BSA and Azide free (ab264111)")
Key features and details
- Mouse monoclonal [Rat 401] to Nestin - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-Nestin antibody [Rat 401] - BSA and Azide free
See all Nestin primary antibodies -
Description
Mouse monoclonal [Rat 401] to Nestin - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse
Does not react with:
Sheep, Cat, Human, Monkey -
Immunogen
Tissue, cells or virus corresponding to Rat Nestin. Homogenized spinal cord tissue from embryonic day 15 (E15) rats.
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Positive control
- WB: Mouse brain and rat brain whole tissue lysates. IHC-P: Rat Brain 6 weeks (cerebellum sagittal, coronal rest) tissue sections. ICC/IF: Mouse embryonic stem cells.
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General notes
ab264111 is the PBS only version of ab6142.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
Rat 401 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat 401] - BSA and Azide free (ab264111)
This image was produced using the same antibody clone, but in a different formulation, ab6142.
IHC image of Nestin staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab6142, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
