Anti-Nestin antibody [4D11] (ab254048)
![Anti-Nestin antibody [4D11] (ab254048)](https://www.abcam.com/ps/products/254/ab254048/Images/ab254048-348693-AntiNestin-antibody-4D11-immunocytochemistry-fluorescence-glial-neurons-rat.jpg "Anti-Nestin antibody [4D11] (ab254048)")
Key features and details
- Mouse monoclonal [4D11] to Nestin
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG1
Overview
-
Product name
Anti-Nestin antibody [4D11]
See all Nestin primary antibodies -
Description
Mouse monoclonal [4D11] to Nestin -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
-
Immunogen
Recombinant fragment within Human Nestin. The exact sequence is proprietary. Expressed in and purified from E. coli.
Database link: P48681 -
Positive control
- ICC/IF: Cultured neonatal rat neurons and glia. WB: Rat brain lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.0325% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
4D11 -
Isotype
IgG1 -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [4D11] (ab254048)
Immunostaining of cultured neonatal rat neurons and glia using ab254048 at 1/500 dilution showing Nestin labeling in red and vimentin in green.
-
![Western blot - Anti-Nestin antibody [4D11] (ab254048)](https://www.abcam.com/ps/products/254/ab254048/Images/ab254048-348692-AntiNestin-antibody-4D11-western-blot-brain-rat.jpg)
Western blot - Anti-Nestin antibody [4D11] (ab254048)Anti-Nestin antibody [4D11] (ab254048) at 1/2000 dilution + Rat brain lysate
