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Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)

Key features and details

  • Mouse monoclonal [10C2] to Nestin - Neural Stem Cell Marker
  • Suitable for: ICC, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Nestin antibody [10C2] - Neural Stem Cell Marker
    See all Nestin primary antibodies

  • Description

    Mouse monoclonal [10C2] to Nestin - Neural Stem Cell Marker

  • Host species

    Mouse

  • Specificity

    Reacts exclusively with human nestin protein.

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Nestin aa 1-1621.
    Database link: P48681

  • Positive control

    • WB: U251 cells. ICC: U251 cells. Human brain tissue.

  • General notes

    Monoclonal antibody 10C2 was derived by fusion of P3x63Ag8.653 myeloma cells with spleen cells from a BALB/c mouse immunized with a 150-amino-acid fragment from the cloned human nestin, conjugated to glutathione S-transferase.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)
    Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) This image is courtesy of an Abreview submitted by Dr Amal Shervington

    ab22035 staining human brain tissue cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 21°C.  The primary antibody was diluted 1/100 and incubated with the samples for 1 hour at 21°C.  A FITC conjugated goat anti-mouse IgG antibody, diluted 1/250, was used as the secondary.

    See Abreview

  • Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)
    Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)

    ICC/IF of U251 cells staining intracellular Nestin filaments with ab22035

  • Western blot - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)
    Western blot - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)
    Lane 1 : Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035) at 1/1000 dilution
    Lane 2 : Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)

    Lane 1 : U251 cells at 10 µl
    Lane 2 : U251 cells at 20 µl

    Predicted band size: 176 kDa


    Exposure time: 60 seconds
  • Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)
    Immunocytochemistry - Anti-Nestin antibody [10C2] - Neural Stem Cell Marker (ab22035)

    Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells using ab22035 (1:100 dilution).

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