Anti-HEPACAM antibody (ab236659)
Key features and details
- Rabbit polyclonal to HEPACAM
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HEPACAM antibody
See all HEPACAM primary antibodies -
Description
Rabbit polyclonal to HEPACAM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human HEPACAM aa 250 to the C-terminus.
Database link: Q14CZ8 -
Positive control
- WB: MCF7 and HepG2 whole cell lysate. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HEPACAM antibody (ab236659)All lanes : Anti-HEPACAM antibody (ab236659) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody (ab236659)MCF7 (human breast adenocarcinoma cell line) cells labeling HEPACAM (green) using ab236659 at 1/200 dilution in ICC/IF. Counterstained with DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
