Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23241-224] to L1CAM - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free
    See all L1CAM primary antibodies

  • Description

    Rabbit monoclonal [EPR23241-224] to L1CAM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate; A375 and MCF7 whole cell lysates. IHC-P: Human kidney, spleen and cerebellum tissue. Flow Cyt: A375 cells. IP: A375 whole cell lysate.

  • General notes

    ab272321 is the carrier-free version of ab270455. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling L1CAM with ab270455 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the molecular layer of the human cerebellum (PMID: 12514225). The section was incubated with ab270455 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

  • Flow Cytometry - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
    Flow Cytometry - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

    Flow cytometric analysis of A549 (Human lung carcinoma epithelial cell, Left) / A375 (Human malignant melanoma epithelial cell, Right) cells labelling L1CAM with ab270455 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    A549 is negative or very low expression cell line for L1CAM (PMID: 23511563).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

  • Immunoprecipitation - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
    Immunoprecipitation - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

    L1CAM was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell), whole cell lysate using ab270455 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270455 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: A375 (human malignant melanoma epithelial cell), whole cell lysate 10ug

    Lane 2: ab270455 IP in A375 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270455 in A375 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling L1CAM with ab270455 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the nerve in human spleen (PMID: 26743472). The section was incubated with ab270455 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling L1CAM with ab270455 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on distal renal tubules in human kidney.The section was incubated with ab270455 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

  • Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
    Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

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