Anti-FLIP antibody (ab233230)
Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FLIP antibody
See all FLIP primary antibodies -
Description
Rabbit polyclonal to FLIP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Rat FLIP aa 1-300. (Expressed in E.coli).
Database link: C0H5Y5 -
Positive control
- WB: Recombinant rat FLIP protein; Jurkat, HeLa and K562 cell lysates; Rat spleen lysate. IHC-P: Rat liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab233230 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLIP antibody (ab233230)
Paraffin-embedded rat liver tissue stained for FLIP using ab233230 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-FLIP antibody (ab233230)Anti-FLIP antibody (ab233230) at 3 µg/ml + Recombinant rat FLIP protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
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Western blot - Anti-FLIP antibody (ab233230)All lanes : Anti-FLIP antibody (ab233230) at 3 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : Rat spleen lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
