Phosphate Assay Kit (Fluorometric) (ab273281)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Serum, Suspension cells, Tissue
Overview
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Product name
Phosphate Assay Kit (Fluorometric)
See all Phosphate kits -
Detection method
Fluorescent -
Sample type
Serum, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Range
0.1 nmol/well - 0.5 nmol/well -
Assay duration
Multiple steps standard assay -
Product overview
Phosphate Assay Kit (Fluorometric) (ab273281) provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate will react with substrate and the Probe to generate fluorescence (Ex/Em = 535/587 nm). The kit can be used to detect Pi in a variety of samples or to monitor phosphate released by an assortment of enzymes (such as ATPases, GTPases, 5’-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase kinase). Unlike other commercially available assays, the assay is not affected by the presence of glucose in samples (Note: Glucose interferes with many other commercially available assays). This assay is highly sensitive with the detection limit of approximately 40 pmol/well.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Converter Enzyme (Lyophilized) 1 vial Developer Enzyme (Lyophilized) 1 vial Phosphate Assay Buffer 1 x 25ml Phosphate Standard (100 mM) 1 x 50µl Probe 1 x 0.4ml Substrate Mix (Lyophilized) 1 vial -
Research areas
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Relevance
Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.
Images
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Phosphate Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.