Human G-CSF ELISA Kit (CSF3)
(ab188390)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.2 pg/ml
- Range: 47 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 7 4.8% Inter-assay Sample n Mean SD CV% Overall 3 5.5% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
5.2 pg/ml -
Range
47 pg/ml - 3000 pg/ml -
Recovery
110 %
Sample specific recovery Sample type Average % Range Cell culture media 110 100% - 116% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human G-CSF ELISA (CSF3) ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency. This version will be discontinued when inventory is depleted. The new version is available as ab264627.
Human G-CSF ELISA Kit (CSF3) (ab188390) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of G-CSF (CSF3)
> protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human G-CSF (CSF3)> with 5.2 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Granulocyte Colony Stimulating Factor (G-CSF) is a glycoprotein that stimulates bone marrow to produce and release granulocytes and stem cells into the bloodstream. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates neutrophil development using Janus kinase (JAK)/signal transducer and activator of transcription (STAT) and Ras/mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human G-CSF Capture Antibody 1 x 600µl 10X Human G-CSF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human G-CSF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions. -
Alternative names
- C17orf33
- Colony stimulating factor 3 (granulocyte)
- CSF 3
see all -
Database links
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
- Unigene: 2233 Human
Images
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Other - Human G-CSF ELISA Kit (CSF3) (ab188390)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of G-CSF standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of PBMC stimulated (+PHA) and unstimulated cell culture supernatants within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Titration of PBMC stimulated (+PHA) and unstimulated cell culture supernatants within the working range of the assay.Bar graph denotes interpolated values of G-CSF protein across dilution series. Dotted line denotes average value for media controls across dilution series.
