Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human G-CSF ELISA Kit, Fluorescent (ab229430)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2 pg/ml
  • Range: 2.93 pg/ml - 12000 pg/ml
  • Sample type: Cell culture supernatant
  • Detection method: Fluorescent
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human G-CSF ELISA Kit, Fluorescent
    See all G-CSF kits

  • Detection method

    Fluorescent

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 4.3%

  • Sample type

    Cell culture supernatant

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2 pg/ml

  • Range

    2.93 pg/ml - 12000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108 104% - 114%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    G-CSF (CSF3) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of G-CSF (CSF3) protein in human cell culture supernatants.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    Granulocyte Colony Stimulating Factor (G-CSF) is a glycoprotein that stimulates bone marrow to produce and release granulocytes and stem cells into the bloodstream. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates neutrophil development using Janus kinase (JAK)/signal transducer and activator of transcription (STAT) and Ras/mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Stoplight Red Substrate 1 x 120µl
    10X Human G-CSF Capture Antibody 1 x 600µl
    10X Human G-CSF Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl
    Antibody Diluent 4BI 1 x 6ml
    Human G-CSF Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated Black 96-Well Microplate 1 unit
    Stoplight Red Substrate Buffer 1 x 12ml

  • Research areas

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

  • Sequence similarities

    Belongs to the IL-6 superfamily.

  • Post-translational
    modifications

    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • C17orf33
    • Colony stimulating factor 3
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3_HUMAN
    • CSF3OS
    • Csfg
    • Filgrastim
    • G-CSF
    • GCSA
    • GCSF
    • Granulocyte colony stimulating factor
    • Granulocyte colony-stimulating factor
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • Pluripoietin
    see all

  • Database links

    Images

    • Catchpoint ELISA Protocol Diagram
      Catchpoint ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human G-CSF (CSF3) standard curve in Sample Diluent NS.
      Example of human G-CSF (CSF3) standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Human G-CSF ELISA Kit, Fluorescent (ab229430)

    © 2021 Astana Biomed Group LLP. All rights reserved.