Anti-PDIA2/PDIP antibody (ab223520)
Key features and details
- Goat polyclonal to PDIA2/PDIP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PDIA2/PDIP antibody
See all PDIA2/PDIP primary antibodies -
Description
Goat polyclonal to PDIA2/PDIP -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
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Immunogen
Tissue, cells or virus corresponding to Human PDIA2/PDIP aa 450 to the C-terminus (internal sequence) (Cysteine residue). NP_006840.2.
Database link: Q13087 -
Positive control
- WB: Human, mouse and rat pancreas lysates. IHC-P: Human pancreas tissue.
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General notes
This product was previously labelled as PDIA2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223520 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA2/PDIP antibody (ab223520)
Paraffin-embedded human pancreas tissue stained for PDIA2/PDIP using ab223520 at 3.8 µg/mL in immunohistochemical analysis, followed by AP-staining.
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Western blot - Anti-PDIA2/PDIP antibody (ab223520)All lanes : Anti-PDIA2/PDIP antibody (ab223520) at 0.1 µg/ml
Lane 1 : Human pancreas lysate (in RIPA buffer)
Lane 2 : Mouse pancreas lysate (in RIPA buffer)
Lane 3 : Rat pancreas lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
