Anti-SMC2 antibody (ab10399)
Key features and details
- Rabbit polyclonal to SMC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMC2 antibody
See all SMC2 primary antibodies -
Description
Rabbit polyclonal to SMC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to SMC2. The epitope maps to a region between residues 50 and 100 of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC2 antibody (ab10399)
Detection of human SMC2 by Western Blot. Samples: Whole cell lysate (90 microgram) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody: ab10399 used at 0.1 ug/ml. Detection: Chemiluminescence with a 10 minute exposure.
Detection of human SMC2 by Western Blot. Samples: Whole cell lysate (90 microgram) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody: ab10399 used at 0.1 ug/ml. Detection: Chemiluminescence with a 10 minute exposure -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (ab10399)IHC image of ab10399 staining in normal human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
