Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bag1 antibody [Y166] (ab32109)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y166] to Bag1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Bag1 antibody [Y166]
    See all Bag1 primary antibodies

  • Description

    Rabbit monoclonal [Y166] to Bag1

  • Host species

    Rabbit

  • Specificity

    The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Bag1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysate and cells, human prostate carcinoma tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Bag1 antibody [Y166] (ab32109)
    Western blot - Anti-Bag1 antibody [Y166] (ab32109)
    Anti-Bag1 antibody [Y166] (ab32109) at 1/500 dilution + HeLa cell lysate

    Predicted band size: 31 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 46 kDa (possible isoform), 50 kDa (possible isoform)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag1 antibody [Y166] (ab32109)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag1 antibody [Y166] (ab32109)

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Bag1 antibody [Y166] (ab32109)
    Immunocytochemistry/ Immunofluorescence - Anti-Bag1 antibody [Y166] (ab32109)
    Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.
  • Anti-Bag1 antibody [Y166] (ab32109)
    Anti-Bag1 antibody [Y166] (ab32109)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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