Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y166] to Bag1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-Bag1 antibody [Y166]
See all Bag1 primary antibodies
Description

Rabbit monoclonal [Y166] to Bag1
Host species

Rabbit
Specificity

The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Bag1 aa 50-150. The exact sequence is proprietary.
Positive control
- HeLa cell lysate and cells, human prostate carcinoma tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Clonality

Monoclonal
Clone number

Y166
Isotype

IgG
Research areas

Western blot - Anti-Bag1 antibody [Y166] (ab32109)

Anti-Bag1 antibody [Y166] (ab32109) at 1/500 dilution + HeLa cell lysate

Predicted band size: 31 kDa
Observed band size: 33 kDa
why is the actual band size different from the predicted?
Additional bands at: 46 kDa (possible isoform), 50 kDa (possible isoform)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag1 antibody [Y166] (ab32109)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Bag1 antibody [Y166] (ab32109)

Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.
Anti-Bag1 antibody [Y166] (ab32109)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com