Human Ferritin ELISA Kit (ab108698)
Key features and details
- Sensitivity: 0.53 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Ferritin ELISA Kit
See all Ferritin Light Chain kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Control 48 Inter-assay Sample n Mean SD CV% Control 48 -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.53 ng/ml -
Recovery
98.66 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Ferritin ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Ferritin (FTL) in serum and plasma.
A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.
Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 10X Washing Solution 1 x 50ml Anti-Ferritin IgG Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Anti-Ferritin-HRP conjugate 1 x 12ml Cover foil 1 unit Ferritin Control 1 x 1ml Ferritin Standard 0 - 0 ng/mL 1 x 3ml Ferritin Standard 1 - 5 ng/mL 1 x 1ml Ferritin Standard 2 - 20 ng/mL 1 x 1ml Ferritin Standard 3 - 100 ng/mL 1 x 1ml Ferritin Standard 4 - 400 ng/mL 1 x 1ml Ferritin Standard 5 - 1,000 ng/mL 1 x 1ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
Involvement in disease
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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Alternative names
- Ferritin L chain
- Ferritin L subunit
- Ferritin light chain
see all -
Database links
- Entrez Gene: 2512 Human
- Omim: 134790 Human
- SwissProt: P02792 Human
- Unigene: 433670 Human
- Unigene: 713706 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108698
