Anti-PLK1 antibody [AZ44] (ab12212)
![Anti-PLK1 antibody [AZ44] (ab12212)](https://www.abcam.com/ps/products/12/ab12212/Images/ab12212_1.jpg "Anti-PLK1 antibody [AZ44] (ab12212)")
Key features and details
- Mouse monoclonal [AZ44] to PLK1
- Suitable for: WB
- Reacts with: Human, Xenopus laevis
- Isotype: IgG1
Overview
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Product name
Anti-PLK1 antibody [AZ44]
See all PLK1 primary antibodies -
Description
Mouse monoclonal [AZ44] to PLK1 -
Host species
Mouse -
Specificity
This is a useful antibody to check that extracts have maintained their CSF status and haven't been activated into interphase. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Xenopus laevis
Does not react with: Mouse -
Immunogen
Recombinant full length protein (Xenopus laevis).
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Epitope
This antibody is thought to bind to a phosphorylated epitope. However, this has not yet been proved, but it does stain in a mitotic specific manner.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AZ44 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PLK1 antibody [AZ44] (ab12212) This image is courtesy of Julian Gannon, CRUK
Western blot using ab12212.
Lane 1: Mitotic CSF Xenopus egg extract
Lane 2: Interphase Xenopus egg extract
Lane 3: HeLa cell extractAZ44 only blots the Xenopus mitotic form of Plx which we assume to be due to this antibody recognizing a phosphorylated epitope.
Western blot using ab12212.Lane 1: Mitotic CSF Xenopus egg extract
Lane 2: Interphase Xenopus egg extract
Lane 3: HeLa cell extract
AZ44 only blots the Xenopus mitotic form of Plx which we assume to be due to this antibody recognizing a phosphorylated epitope.
