Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5715] to Nucleoside phosphorylase
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Nucleoside phosphorylase antibody [EPR5715]
    See all Nucleoside phosphorylase primary antibodies

  • Description

    Rabbit monoclonal [EPR5715] to Nucleoside phosphorylase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in Human Nucleoside Phosphorylase.

  • Positive control

    • HeLa, Jurkat, JAR, K562, Human placenta, and 293T lysates. ICC/IF: SH SY5Y cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.76 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5715

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PNP knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 32 kDa
    Observed band size: 31 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab109447 observed at 31 kDa. Red - loading control ab7291 observed at 50 kDa. 

     ab109447 Anti-Nucleoside phosphorylase antibody [EPR5715]  was shown to specifically react with Nucleoside phosphorylase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266158 (knockout cell lysate ab257594) was used.  Wild-type and Nucleoside phosphorylase knockout samples were subjected to SDS-PAGE.  ab109447 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    ab109447 at 1/100 dilution staining Nucleoside phosphorylase in SH SY5Y cells by Immunofluorescence.
  • Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : JAR lysate
    Lane 3 : K562 lysate
    Lane 4 : Human placenta lysate
    Lane 5 : 293T lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 32 kDa
    Observed band size: 31 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    OI-RD Scanning - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
    Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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