Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
![Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-387023-anti-bst2tetherin-antibody-epr23597266-western-blot-mouse-spleen-mouse-thymusmouse-lymph-node-p388d1-lysates.jpg "Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23597-266] to BST2/Tetherin
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse
Overview
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Product name
Anti-BST2/Tetherin antibody [EPR23597-266]
See all BST2/Tetherin primary antibodies -
Description
Rabbit monoclonal [EPR23597-266] to BST2/Tetherin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF MouseIP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen, thymus and lymph node tissue lysates; P388D1 whole cell lysate. ICC/IF: Mouse splenocytes. Flow Cyt: Mouse splenocytes. IP: Mouse spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23597-266 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)All lanes : Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse thymus tissue lysate
Lane 3 : Mouse lymph node tissue lysate
Lane 4 : P388D1 (mouse lymphoma monocyte; macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 28-40,64 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, and forms cysteine-linked dimers at 64 KD which is consistent to the literature(PMID: 22520941; PMID: 19737401).
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![Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-1-anti-bst2tetherin-antibody-epr23597266-immunocytochemistry-mouse-splenocytes.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Mouse splenocyte cells labelling BST2/Tetherin with ab272169 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in mouse splenocytes. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.
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![Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-2-anti-bst2tetherin-antibody-epr23597266-flow-cytometry-mouse-splenocytes.jpg)
Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)Flow cytometric analysis of Mouse splenocyte cells labelling BST2/Tetherin with ab272169 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (AlexaFluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab272169 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.
Gated on viable cells.
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![Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-3-anti-bst2tetherin-antibody-epr23597266-immunoprecipitation-mouse-spleen-tissue-lysate-10ug.jpg)
Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)BST2/Tetherin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab272169 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272169 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse spleen tissue lysate 10ug
Lane 2: ab272169 IP in Mouse spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272169 in mouse spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).
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![Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-5-benefits-of-recombinant-antibodies.png)
Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
