Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23597-202] to BST2/Tetherin - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free
    See all BST2/Tetherin primary antibodies

  • Description

    Rabbit monoclonal [EPR23597-202] to BST2/Tetherin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen tissue. ICC/IF: EL.4 and mouse splenocyte cells. Flow Cyt: Mouse splenocytesl. IP: Mouse spleen tissue lysate; EL4 whole cell lysate.

  • General notes

    ab272175 is the carrier-free version of ab246508. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling BST2/Tetherin with ab246508 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen (PMID: 19903902).The section was incubated with ab246508 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Mouse splenocyte cells labelling BST2/Tetherin with ab246508 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in mouse splenocyte ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
    Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

    Flow cytometric analysis of  Mouse splenocyte cells labelling BST2/Tetherin with ab246508 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab246508 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
    Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

    BST2/Tetherin was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte), whole cell lysate with ab246508 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246508 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug

    Lane 2: ab246508 IP in EL4 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246508 in EL4 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

    Immunofluorescent analysis of 100% methanol-fixed EL.4 cells labelling BST2/Tetherin with ab246508 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in EL.4 cell line. 100% methanol fixation is recommended. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
    Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

    BST2/Tetherin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab246508 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246508 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen tissue lysate 10ug

    Lane 2: ab246508 IP in Mouse spleentissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246508 in mouse spleen tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling BST2/Tetherin with ab246508 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse skeletal muscle (PMID: 19903902). The section was incubated with ab246508 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

  • Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
    Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

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