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Anti-RAB29 antibody [MJF-R30-124] (ab256526)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R30-124] to RAB29
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RAB29 antibody [MJF-R30-124]
    See all RAB29 primary antibodies

  • Description

    Rabbit monoclonal [MJF-R30-124] to RAB29

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild type A549, A549, HEK293T cells, HEK293T cells, wildtype C57BL6 mouse lung and Rab29 knock-out C57BL6 mouse lung lysates. IP: A549 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    MJF-R30-124

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)
    Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)
    All lanes : Anti-RAB29 antibody [MJF-R30-124] (ab256526) at 1/3000 dilution

    Lane 1 : wildtype C57BL6 mouse lung tissue lysate
    Lane 2 : Rab29 knock-out C57BL6 mouse lung tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : IR Dye 800CW Goat anti-rabbit IgG
    (#926-32211) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    This antibody reacts with unidentifiable proteins around 30,75 and 150 kDa.  This image was kindly provided by Dr. Mark Cookson, NIH.

    Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer.

    Exposure Time: 3 minutes.

  • Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)
    Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)
    All lanes : Anti-RAB29 antibody [MJF-R30-124] (ab256526) at 1/3000 dilution

    Lane 1 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with non-target siRNA control whole cell lysate
    Lane 2 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with siRNA against Rab29 whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : IR Dye 800CW Goat anti-rabbit IgG
    (#926-32211) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    This antibody reacts with an unidentifiable protein around 150 kDa.  This image was kindly provided by Dr. Mark Cookson, NIH.

    Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer 

    Exposure Time: 3 min

  • Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)
    Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)
    All lanes : Anti-RAB29 antibody [MJF-R30-124] (ab256526) at 1/1000 dilution

    Lane 1 : Wild type A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : A549 (human lung carcinoma epithelial cell) Rab29 KO whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    The lysates were kindly provided by Dr. Dario Alessi, University of Dundee.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    Exposure Time: 3 min

  • Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] (ab256526)
    Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] (ab256526)

    RAB29 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab256526 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256526 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab256526 IP in A549 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256526 in A549 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 30 seconds

     

  • Anti-RAB29 antibody [MJF-R30-124] (ab256526)
    Anti-RAB29 antibody [MJF-R30-124] (ab256526)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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