Antibodies, Proteins, Kits and Reagents for Life Science

53 606 ₸ Product size

10 µl 53 606 ₸ 100 µl 154 118 ₸

Order now and get it on Tuesday March 09, 2021

Anti-RAB29 antibody [MJF-R30-124] (ab256526)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [MJF-R30-124] to RAB29
Suitable for: IP, WB
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-RAB29 antibody [MJF-R30-124]
See all RAB29 primary antibodies
Description

Rabbit monoclonal [MJF-R30-124] to RAB29
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild type A549, A549, HEK293T cells, HEK293T cells, wildtype C57BL6 mouse lung and Rab29 knock-out C57BL6 mouse lung lysates. IP: A549 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

MJF-R30-124
Isotype

IgG
Research areas

Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)

All lanes : Anti-RAB29 antibody [MJF-R30-124] (ab256526) at 1/3000 dilution

Lane 1 : wildtype C57BL6 mouse lung tissue lysate
Lane 2 : Rab29 knock-out C57BL6 mouse lung tissue lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : IR Dye 800CW Goat anti-rabbit IgG
(#926-32211) at 1/10000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa

This antibody reacts with unidentifiable proteins around 30,75 and 150 kDa. This image was kindly provided by Dr. Mark Cookson, NIH.

Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer.

Exposure Time: 3 minutes.
Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)

All lanes : Anti-RAB29 antibody [MJF-R30-124] (ab256526) at 1/3000 dilution

Lane 1 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with non-target siRNA control whole cell lysate
Lane 2 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with siRNA against Rab29 whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : IR Dye 800CW Goat anti-rabbit IgG
(#926-32211) at 1/10000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa

This antibody reacts with an unidentifiable protein around 150 kDa. This image was kindly provided by Dr. Mark Cookson, NIH.

Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer

Exposure Time: 3 min
Western blot - Anti-RAB29 antibody [MJF-R30-124] (ab256526)

All lanes : Anti-RAB29 antibody [MJF-R30-124] (ab256526) at 1/1000 dilution

Lane 1 : Wild type A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell) Rab29 KO whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Predicted band size: 23 kDa
Observed band size: 23 kDa

The lysates were kindly provided by Dr. Dario Alessi, University of Dundee.

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure Time: 3 min
Immunoprecipitation - Anti-RAB29 antibody [MJF-R30-124] (ab256526)

RAB29 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab256526 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256526 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug

Lane 2: ab256526 IP in A549 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256526 in A549 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST

Exposure time: 30 seconds
Anti-RAB29 antibody [MJF-R30-124] (ab256526)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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