Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free
    See all RAB29 primary antibodies

  • Description

    Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C] whole cell lysate.

  • General notes

    Ab243289 is the carrier-free version of ab241062. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243289 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)
    Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)
    All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/500 dilution

    Lane 1 : MLi-2 (100nM for 60min) treated HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
    Lane 2 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
    Lane 3 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 1 µg
    Lane 4 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.3 µg
    Lane 5 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.1 µg

    Secondary
    All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241062).

  • Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)
    Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)

    Dot blot analysis of RAB29 (phospho T71) labeled with ab241062 at 1/1000 dilution.

    Lane 1: RAB29 (phospho T71) peptide.
    Lane 2: RAB29 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241062).
  • Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)
    Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] - BSA and Azide free (ab243289)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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